TRF1 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-trf1-antibody-vma00032.html?RenderPageSpecific=1
TRF1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with TRF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to cytokeratin 8 (molecular weight 60 kDa)

Enlarge
  • TRF1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • TRF1 Antibody with Control Lysate
  • Mouse anti TRF1
(Rated 0.0 out of 5 based on 0 customer reviews)
    TRF1 antibody recognizes telomeric repeat-binding factor 1, also known as NIMA-interacting protein 2, TTAGGG repeat-binding factor 1 or telomeric protein Pin2/TRF1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00032WBdatasheet pdfdatasheet pdf100 µl
      VMA00032
      VMA00032KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00032KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human TRF1 antibody recognizes human telomeric repeat-binding factor 1, also known as TTAGGG repeat binding factor 1 (TRF1) or NIMA-interacting protein 2. TRF1 is a ubiquitously expressed 439 amino acid DNA binding protein and negative regulator of telomere length containing a single Myb domain. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited.

        Additionally, in conjunction with POT1 and TTAGGG repeat binding factor 2 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1 and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage (Wellinger 2009). TRF1 is thought to play a role in leukaemia (Shi et al. 2006).

        Mouse anti Human TRF1 antibody recognizes human TRF1 as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "TRF1" specificity.