TPT1 Antibody | 3C7

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TPT1 Antibody | 3C7 gallery image 1

Immunofluorescence showing TPT1 in HeLa cells using Mouse anti Human TPT1:Azide Free (MCA4181Z)

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TPT1 Antibody | 3C7 gallery image 2

Western blot analysis of TPT1 expression in HepG2 using Mouse anti Human TPT1:Azide Free (MCA4181Z)

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  • TPT1 Antibody | 3C7 thumbnail image 1
  • TPT1 Antibody | 3C7 thumbnail image 2
  • Mouse anti Human TPT1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3C7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4181ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4181Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human TPT1 antibody, clone 3C7 recognizes human Translationally-controlled tumor protein, also known as TPT1, TCTP, Fortilin, Histamine-releasing factor, HRF or p23. TPT1 is a 172 amino acid ~20 kDa protein involved in calcium binding and microtubule stabilization. TPT1 is expressed in many normal cell types including erythrocytes, hepatocytes, macrophages and platelets. It is also detectable in several tumor cell types including erythroleukemia, glioma, melanoma and lymphoma (Sanchez et al. 1997).

      Two isoforms of TPT1 generated by alternative splicing have been identified, both containing the the peptide sequence of the immunogen used to generate clone 3C7. Mouse anti Human TPT1 antibody, clone 3C7 is therefore expected to recognize both potential isoforms of TPT1 (UniProt : P13693).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 35-139 of human TPT1
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein binding
      calcium ion binding
      anti-apoptosis
      calcium ion transport
      response to virus
      cellular calcium ion homeostasis
      multivesicular body
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TPT1 Antibody Formats

    Formats Clone Applications Sizes available
    TPT1 Antibody : Preservative Free 3C7 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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