TOMM22 Antibody | 4G4

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TOMM22 Antibody | 4G4 gallery image 1

Immunofluorescence showing TOMM22 in HeLa cells using Mouse anti Human TOMM22:Azide Free (MCA3894Z)

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TOMM22 Antibody | 4G4 gallery image 2

Staining of paraffin embedded human small intestine with Mouse anti Human TOMM22:Azide Free (MCA3894Z)

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TOMM22 Antibody | 4G4 gallery image 3

Western blot analysis of TOMM22 expression in NIH/3T3 using Mouse anti Human TOMM22:Azide Free (MCA3894Z)

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  • TOMM22 Antibody | 4G4 thumbnail image 1
  • TOMM22 Antibody | 4G4 thumbnail image 2
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  • Mouse anti Human TOMM22:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4G4
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3894ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3894Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human TOMM22 antibody, clone 4G4 recognizes human Mitochondrial import receptor subunit TOM22 homolog, also known as TOMM22, 1C9-2 or translocase of outer membrane 22 kDa subunit homolog. TOM22 is a 141 amino acid ~22 kDa single pass type 1 outer mitochondrial transmembrane protein, receptor component of the TOM complex involved in translocation of mitochondrial preproteins (Yano et al.2000).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-143 of human TOMM22
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane of membrane fraction
      protein import into mitochondrial outer membrane
      receptor activity
      protein transmembrane transporter activity
      integral to membrane
      mitochondrial outer membrane translocase complex
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TOMM22 Antibody Formats

    Formats Clone Applications Sizes available
    TOMM22 Antibody : Preservative Free 4G4 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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