TNF Alpha Antibody | D2E7

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Purity assessment by SDS-PAGE

0.5 μg of Human Anti-TNFα Antibody, MCA6088 (adalimumab biosimilar, research grade) and adalimumab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie Blue staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.

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Monodispersity assessment by HPLC-SEC

Size exclusion chromatography (SEC) of Human Anti-TNFα Antibody, MCA6088 (adalimumab biosimilar, research grade) and adalimumab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl; flow rate: 200 μl/min; inj. volume [μl]: 2; temp.: 25 °C; detection: 280 nm; sample: mAb (0.5-5 mg/ml).

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Adalimumab bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Adalimumab Antibody (clone AbD18654, HCA202) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of adalimumab (therapeutic reference product) or Human Anti-TNFα Antibody, MCA6088 (adalimumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Adalimumab Antibody (clone AbD18654_hIgG1, HCA203) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®

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  • Human anti TNF Alpha (Adalimumab Biosimilar)
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-TNF Alpha Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug adalimumab (Humira) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA6088Edatasheet pdfdatasheet pdf0.2 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human Anti-TNF alpha Antibody is a research grade biosimilar of the monoclonal antibody drug adalimumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic adalimumab antibody clone D2E7.

        This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-adalimumab antibodies, for example HCA202 and HCA203. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-adalimumab antibody in IgG1 format as reference standard, for example HCA203, HCA204 or HCA205, or an anti-adalimumab antibody in IgE format as reference standard, for example HCA271.

        The therapeutic antibody adalimumab (Humira) is a fully human monoclonal antibody (IgG1 kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNF alpha, and acts by blocking the activation of TNF receptors by TNF alpha. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        This product is NOT FOR THERAPEUTIC USE.

        View a summary of supporting anti-adalimumab antibody products
      • Intended Use
      • Target Species
      • Product Form
        Human IgG1 (kappa) antibody - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.02% Proclin 300
      • Immunogen
        Human TNFα
      • Purity
        >98% by SDS PAGE
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
      • Licensed Use
        For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional TNF Alpha Antibody Formats

      Formats Clone Applications Sizes available
      TNF Alpha Antibody : Purified D2E7 E 0.2 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Adalimumab:HRPHCA204P0.1 mgE
            Human anti Adalimumab (Drug/Target Complex):HRPHCA232P0.1 mgE
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            Human anti AdalimumabHCA2020.1 mgE
            Human anti AdalimumabHCA2030.1 mgE
            Human anti AdalimumabHCA2040.1 mgE
            Human anti AdalimumabHCA2050.1 mgE
            Human anti Adalimumab (Drug/Target Complex)HCA2060.1 mgE
            Human anti Adalimumab (Drug/Target Complex)HCA2070.1 mgE
            Human anti Adalimumab (Drug/Target Complex)HCA2310.1 mgE
            Human anti Adalimumab (Drug/Target Complex)HCA2320.1 mgE
            Human anti AdalimumabHCA2710.1 mgE
            Hispec Assay DiluentBUF049A50 mlIY

            Recommended Positive Controls

              Histology Controls

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