TNF Alpha Antibody | 4C6-H6

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  • Mouse anti Human TNF Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4C6-H6
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1385E, F *, IP, Pdatasheet pdfdatasheet pdf2 ml
    MCA1385
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human TNF alpha antibody clone 4C6-H6 recognizes human Tumor necrosis factor, also known as TNF alpha, Cachectin or Tumor necrosis factor ligand superfamily member 2. TNFα is a 233 amino acid ~22kDa type II transmembrane protein with a single potential glycosylation site (Uniprot P01375: Takakura-Yamamoto et al. 1996).

      Mouse anti Human TNF alpha antibody clone 4C6-H6 has been used successfully for the demonstration of TNFα expression in formalin fixed, paraffin embedded bone marrow biopsies from patients with Myelodysplastic syndrome (Molnár et al. 2000) ant has been used for evaluating the increase in TNFα expression seen in peripheral blood mononuclear cells from patients suffering from the autoinnmuse condition, Graves disease (Quadbeck et al. 2006).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/21/10
      Immunohistology - Paraffin
      Immunoprecipitation
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TNF Alpha Antibody Formats

    Formats Clone Applications Sizes available
    TNF Alpha Antibody : S/N 4C6-H6 E, F *, IP, P 2 ml
    • Copyright © 2016 Bio-Rad

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      STAR86PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF
        MCA692

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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