TMS1 Antibody (PrecisionAbTM Antibody) | OTI1A2

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TMS1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1A2 gallery image 1

Western blot analysis of whole cell lysates probed with TMS1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 120 second exposure. Arrow points to TMS1 (molecular weight 22 kDa)

  • TMS1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1A2 thumbnail image 1
  • Mouse anti TMS1
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    TMS1 antibody recognizes apoptosis-associated speck-like protein containing a CARD, also known as caspase recruitment domain-containing protein 5 or target of methylation-induced silencing 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00183WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Mouse anti Human TMS1 antibody recognizes target of methylation-induced silencing 1 (TMS1), also known as apoptosis-associated speck-like protein containing a CARD and caspase recruitment domain-containing protein 5.

        The TMS1 (PYCARD) gene encodes TMS1, an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for PYCARD (provided by RefSeq, Jul 2008).

        Mouse anti Human TMS1 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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