Titin Antibody | 2B3

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Titin Antibody | 2B3 gallery image 1

Staining of paraffin-embedded human skeletal muscle with Mouse anti Human TITIN (MCA5176Z)

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  • Mouse anti Human Titin:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2B3
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5176ZPdatasheet pdfdatasheet pdf0.1 mg
    MCA5176Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Titin antibody, clone 2B3 recognizes human Titin, also known as connectin or Rhabdomyosarcoma antigen MU-RMS-40.14. Titin is a 34350 amino acid ~ 3,820 kDa essential component of striated muscle contributing to the balance of forces across individual sarcomeres.

      Multiple isoforms of titin caused by alternative splicing have been described. All described isoforms have a complete unaltered c-terminal region and Mouse anti Human Titin antibody, clone 2B3 binds to all described isoforms of human titin.

      Mutations in the titin gene can lead to the a range of myopathic conditions including Hereditary myopathy with early respiratory failure (HMERF), Cardiomyopathy, familial hypertrophic 9 (CMH9), Cardiomyopathy, dilated 1G (CMD1G), Tardive tibial muscular dystrophy (TMD), Limb-girdle muscular dystrophy 2J (LGMD2J) and Early-onset myopathy with fatal cardiomyopathy (EOMFC).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1 - 111 of human TITIN
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Titin Antibody Formats

    Formats Clone Applications Sizes available
    Titin Antibody : Preservative Free 2B3 P 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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