Tissue Transglutaminase Antibody

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Tissue Transglutaminase Antibody gallery image 1

Western blot analysis of cell lysates using Rabbit anti tissue transglutaminase antibody

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Tissue Transglutaminase Antibody gallery image 2

Immunohistochemistry of paraffin-embedded human prostate using Rabbit anti tissue transglutaminase antibody at a dilution of 1/100

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Tissue Transglutaminase Antibody gallery image 3

Immunohistochemistry of paraffin-embedded human stomach cancer using Rabbit anti tissue transglutaminase antibody at a dilution of 1/100

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Tissue Transglutaminase Antibody gallery image 4

Immunohistochemistry of paraffin-embedded mouse brain using Rabbit anti tissue transglutaminase antibody

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Tissue Transglutaminase Antibody gallery image 5

Immunohistochemistry of paraffin-embedded human lung cancer using Rabbit anti tissue transglutaminase antibody

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Tissue Transglutaminase Antibody gallery image 6

Immunohistochemistry of paraffin-embedded human esophagus using Rabbit anti tissue transglutaminase antibody

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Tissue Transglutaminase Antibody gallery image 7

Immunohistochemistry of paraffin-embedded human stomach using Rabbit anti tissue transglutaminase antibody

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  • Rabbit anti Tissue Transglutaminase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2532P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2532
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti tissue transglutaminase antibody recognizes tissue transglutaminase, also known as protein-glutamine gamma-glutamyltransferase 2. Tissue transglutaminase belongs to the transglutaminase family of proteins and is an enzyme which catalyzes protein cross-linking and addition of polyamines to proteins.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to tissue transglutaminase was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Recombinant human tissue transglutaminase
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      metal ion binding
      apoptotic cell clearance
      positive regulation of cell adhesion
      acyltransferase activity
      protein-glutamine gamma-glutamyltransferase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/1001/200
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti tissue transglutaminase antibody detects a band of approximately 90 kDa in cell lysates under reducing conditions
    • Instructions For Use

    Additional Tissue Transglutaminase Antibody Formats

    Formats Applications Sizes available
    Tissue Transglutaminase Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
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      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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