TIMP-1

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  • Recombinant Human TIMP-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP268E, FN, WBdatasheet pdfdatasheet pdf10 µg
    PHP268
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), is a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. Studies have shown that a correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 100ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% BSa is recommended.
    • Preparation
      Purified recombinant TIMP-1 expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      TIMP-1 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-1 concentration of approximately 0.5 μg/ml and MMP-1 concentration of 1.6 μg/ml.
    • Purity
      >95% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Molecular Weight
      20.6kD (184 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
      10mM Sodium Phosphate, pH7.5.
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      platelet alpha granule lumen
      negative regulation of membrane protein ectodomain proteolysis
      positive regulation of cell proliferation
      metal ion binding
      platelet activation
      metalloendopeptidase inhibitor activity
      proteinaceous extracellular matrix
      platelet degranulation
      protein binding
      erythrocyte maturation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays5.010.0ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PHP268 may be used as a standard in ELISA applications involving AHP2187 or AHP2187B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      PHP268 may be used as a positive control for western blotting applications involving AHP2187 or AHP2187B.
    • Instructions For Use

    Additional TIMP-1 Formats

    Formats Applications Sizes available
    TIMP-1 : Rec. Protein E, FN, WB 10 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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