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TIMP-1 Antibody

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  • Rabbit anti Human TIMP-1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9013-1108E, P, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human TIMP-1 antibody recognizes human Tissue inhibitor of metalloproteinases 1, also known as TIMP-1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity or Fibroblast collagenase inhibitor. TIMP-1 is a 207 amino acid, ~23 kDa secreted glycoprotein containing a 23 amino acid signaling peptide and a single NTR domain. TIMP-1 is a metalloproteinase inhibitor that functions by formation of complexes with target metalloproteinases, binding to their catalytic cofactor leading to irreversible inactivation of the target metalloproteinase. TIMP-1 also functions as a growth factor and activates signalling cascades via CD63 and CD29 (Uniprot: P01033). In humans TIMP-1 also functions to promote growth and differentiation of erythroid progenitors (Gasson et al. 1985).

      Rabbit anti Human TIMP-1 antibody recognizes both native and denatured TIMP-1, as well as TIMP-1 in and out of enzyme/inhibitor complex. This antibody is not known to be neutralizing.TIMP-1 is expressed by a wide range of cells including microvascular endothelial cells, smooth muscle cells, mesangial cells and some fibroblasts.

      Rabbit anti Humn TIMP-1 antibody has been used successfully for the localization of TIMP-1 in a rabbit model of hypertrophic scarring by immunohistochemistry on formalin fixed, paraffin embedded sections (Tark et al. 2015).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.02%Sodium Azide (NaN3)
    • Immunogen
      Human tissue inhibitor of metalloproteinase 1 (TIMP-1).
    • Purity
    • Approx. Protein Concentrations
      1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      metal ion binding
      metalloendopeptidase inhibitor activity
      platelet alpha granule lumen
      negative regulation of membrane protein ectodomain proteolysis
      platelet degranulation
      erythrocyte maturation
      positive regulation of cell proliferation
      proteinaceous extracellular matrix
      protein binding
      platelet activation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA2.5 µg/ml10 µg/ml
      Immunohistology - Paraffin1 µg/ml5 µg/ml
      Western Blotting5 µg/ml10 µg/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TIMP-1 Antibody Formats

    Formats Applications Sizes available
    TIMP-1 Antibody : Purified E, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls


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