Thymidylate Synthase antibody | OTI9H4
IP-MS Validated
Mouse anti Thymidylate Synthase
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI9H4
- Isotype
- IgG1
- Format
- Purified
- Specificity
- Thymidylate Synthase
Antibody Quality and Performance Guarantee
| Mouse anti Human thymidylate synthase antibody, clone OTI9H4 recognizes thymidylate synthase, also known as TSase. Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of TYMS and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase (provided by RefSeq, Jul 2008). Mouse anti Human thymidylate synthase antibody, clone OTI9H4 detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti thymidylate synthase detects a band of approximately 30 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human thymidylate synthase (NP_001062) produced in SF9 cells
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P04818
- Entrez Gene
- TYMS
- GO Terms
- GO:0006260 DNA replication
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0005829 cytosol
- GO:0004799 thymidylate synthase activity
- GO:0006206 pyrimidine base metabolic process
- GO:0006281 DNA repair
- GO:0046683 response to organophosphorus
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0046134 pyrimidine nucleoside biosynthetic process
- View More GO Terms
VMA00217
061015
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