Thrombopoietin

//www.bio-rad-antibodies.com/human-thrombopoietin-recombinant-protein-php086a.html?RenderPageSpecific=1
Thrombopoietin gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • Thrombopoietin thumbnail image 1
  • Recombinant Human Thrombopoietin
  • Recombinant Human Thrombopoietin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP086BFNdatasheet pdfdatasheet pdf10 µg
    PHP086B
    PHP086AFNdatasheet pdfdatasheet pdf2 µg
    PHP086A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human Thrombopoietin is an E.coli derived protein corresponding to human thrombopoietin (TPO), also known as the Mp1-ligand, MGDF. TPO stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. This preparation of Recombinant Human Thrombopoietin comprises the receptor binding domain of Mpl-ligand protein. ED50 <1.0ng/ml determined by dose-dependent stimulation of M07e cells, corresponding to a specific activity of > 1 x 106 units/mg.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Pack Size: 10 µgReconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 2 µgReconstitute with 20 ul distilled water. Recombinant Human Thrombopoietin
      Pack Size: 10 µgReconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 2 µgReconstitute with 20 ul distilled water. Recombinant Human Thrombopoietin
    • Preparation
    • Preservative Stabilisers
      None present
      None present
    • Purity
      >98% by SDS PAGE and HPLC analysis
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      Pack Size: 10 µgTotal protein concentration 0.1 mg/ml after reconstitution.
      Pack Size: 2 µg0.1 mg/ml after reconstitution
      Pack Size: 10 µgTotal protein concentration 0.1 mg/ml after reconstitution.
      Pack Size: 2 µg0.1 mg/ml after reconstitution
    • Protein Molecular Weight
      18.6kDa (174 amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      Pack Size: 10 µg10mM Sodium Citrate
      Pack Size: 2 µg0.1% Trifluoroacetic acid
      Pack Size: 10 µg10mM Sodium Citrate
      Pack Size: 2 µg0.1% Trifluoroacetic acid
    • Storage
      Pack Size: 10 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 2 µgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 10 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 2 µgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
      3 months from date of reconstitution.
    • GO Terms
      growth factor activity
      extracellular space
      cytokine activity
      cell proliferation
      platelet activation
      hormone activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Thrombopoietin Formats

    Formats Applications Sizes available
    Thrombopoietin : Rec. Protein FN 10 µg | 2 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...