Thrombopoietin

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  • Recombinant Human Thrombopoietin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP086AFNdatasheet pdfdatasheet pdf2 µg
    PHP086A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human Thrombopoietin is an E.coli derived protein corresponding to human thrombopoietin (TPO), also known as the Mp1-ligand, MGDF. TPO stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. This preparation of Recombinant Human Thrombopoietin comprises the receptor binding domain of Mpl-ligand protein. ED50 <1.0ng/ml determined by dose-dependent stimulation of M07e cells, corresponding to a specific activity of > 1 x 106 units/mg.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Reconstitute with 20 ul distilled water. Recombinant Human Thrombopoietin
    • Preparation
    • Preservative Stabilisers
      None present
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1 mg/ml after reconstitution
    • Protein Molecular Weight
      18.6kDa (174 amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      0.1% Trifluoroacetic acid
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      growth factor activity
      extracellular space
      cytokine activity
      cell proliferation
      platelet activation
      hormone activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Thrombopoietin Formats

    Formats Applications Sizes available
    Thrombopoietin : Rec. Protein FN 2 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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