Thioredoxin Antibody | 2B1

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Thioredoxin Antibody | 2B1 gallery image 1

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Mouse anti Human thioredoxin antibody, clone 2B1 (MCA1538) used to assess its ability to to inhibit HIV infection of primary human monocyte derived macrophages in vitro
Image caption:
Inhibition of thiol-disulfide exchange suppresses HIV-1 infection of primary human MDM. After preincubation for 30 minutes. with various concentrations of DTNB, anti-PDI or anti-Trx mAbs, MDM were infected (in duplicate, in the presence of the inhibitors) for 4 h with the HIV-1 strains indicated, washed twice, and then maintained in MØ medium without the reagents. Cell culture supernatants were harvested and replenished (80% v/v) every three days, and harvested supernatants were stored at -80°C before being analyzed for reverse transcriptase (RT) activity.

From: Stantchev TS, Paciga M, Lankford CR, Schwartzkopff F, Broder CC, Clouse KA.
Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection.
Retrovirology. 2012 Dec 3;9:97.

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Thioredoxin Antibody | 2B1 gallery image 2

Published customer image:
Mouse anti Human thioredoxin antibody, clone 2B1 (MCA1538) used to assess its ability to inhibit HIV infection of PM-1 T-cells. in vitro
Image caption:
Anti-PDI,but not anti-Thioredoxin mAbs inhibit HIV-1 infection in PM-1 T-cell line.A. PM-1 cells were pre-incubated for 1 h in RPMI-1640 containing the indicated concentrations of nonspecific mouse IgG, anti-PDI or anti-Trx mAbs prior to infection with HIV-1JR-FL Env pseudotyped, Luc reporter gene-encoding virus particles for 2.5 h in the presence of the different mAbs. After the infection period, cells were washed and further incubated for 48 h in RC-10 before being lysed with 0.5% Triton-X100 in PBS and Luc activity was measured. B, C. Following preincubation for 30 minutes with 5 mM DTNB, anti-PDI or anti-Trx mAbs, PM-1cells were infected with HIV-1ADA (panel B) or HIV-1LAV (panel C). After infection for 2 h in the presence of the aforementioned inhibitors, the PM-1 cells were washed and further incubated in RC-10 without DTNB or mAbs. Cell culture supernatants were collected every two days and cryopreserved before being evaluated for RT activity.

From: Stantchev TS, Paciga M, Lankford CR, Schwartzkopff F, Broder CC, Clouse KA.
Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection.
Retrovirology. 2012 Dec 3;9:97.

Enlarge
Thioredoxin Antibody | 2B1 gallery image 3

Published customer image:
Mouse anti Human thioredoxin antibody, clone 2B1 (MCA1538) used to assess thioredoxin expression levels on different cell types in culture by flow cytometry.
Image caption:
Cell surface expression of PDI and/or Thioredoxin. PM-1 T cells (panel A), primary human MDM (panel B) and PBLs (panel C) were isolated and cultured as described in Methods. Subsequently, the cells were fixed with 1.6% paraformaldehyde and treated with NH4Cl to quench the reactive aldehyde groups before labeling. The PDI and Trx levels on the cell surface were detected by a combination of directly labeled anti-PDI-FITC and anti-Trx-APC mAbs from the same clones used in the HIV-1 infection experiments. In addition, the unstimulated and PHA-P stimulated PBLs were labeled with anti-CD4-Pacific blue conjugated mAb, and the PDI and Trx expression were analyzed on the CD4+ lymphocyte (Ly) population. The results were analyzed and the final graphs were created using FlowJoe software.

From: Stantchev TS, Paciga M, Lankford CR, Schwartzkopff F, Broder CC, Clouse KA.
Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection.
Retrovirology. 2012 Dec 3;9:97.

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  • Mouse anti Human Thioredoxin
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  • Product Type
    Monoclonal Antibody
  • Clone
    2B1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1538C, E, P, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1538
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human thioredoxin antibody, clone 2B1 recognizes human thioredoxin, also known as ATL-derived factor or Surface-associated sulphydryl protein. Thioredoxin is a 105 amino acid ~12kDa dithiol oxidoreductase with powerful protein disulphide oxidoreductase activity containing a single thioredoxin domain (UniProt: P10599). Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB (Kelleher et al. 2014). Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells (Rubartelli et al. 1992).

      Mouse anti Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells (Di Trapani et al. 1998), a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting. Mouse anti Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by imunohistochemistry (Lincoln et al. 2003). Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin (Perking et al. 1995)
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting10ug/ml50ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Thioredoxin Antibody Formats

    Formats Clone Applications Sizes available
    Thioredoxin Antibody : Purified 2B1 C, E, P, WB 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              References

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