Thioredoxin Antibody | 2B1

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  • Mouse anti Human Thioredoxin
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  • Product Type
    Monoclonal Antibody
  • Clone
    2B1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1538C, E, P, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1538
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human thioredoxin antibody, clone 2B1 recognizes human thioredoxin, also known as ATL-derived factor or Surface-associated sulphydryl protein. Thioredoxin is a 105 amino acid ~12kDa dithiol oxidoreductase with powerful protein disulphide oxidoreductase activity containing a single thioredoxin domain (UniProt: P10599). Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB (Kelleher et al. 2014). Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells (Rubartelli et al. 1992).

      Mouse anti Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells (Di Trapani et al. 1998), a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting. Mouse anti Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by imunohistochemistry (Lincoln et al. 2003). Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin (Perking et al. 1995)
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting10ug/ml50ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Thioredoxin Antibody Formats

    Formats Clone Applications Sizes available
    Thioredoxin Antibody : Purified 2B1 C, E, P, WB 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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