Thioredoxin 1 Antibody (PrecisionAbTM Antibody)

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Thioredoxin 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with thioredoxin 1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to thioredoxin 1 (molecular weight 12 kDa)

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  • Thioredoxin 1 Antibody with Control Lysate
  • Mouse anti Thioredoxin 1
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2b
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00585WBdatasheet pdfdatasheet pdf100 µl
    VMA00585
    VMA00585KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00585KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Mouse anti Human thioredoxin 1 antibody recognizes thioredoxin, also known as ADF, ATL-derived factor, SASP, TRX or TXN delta 3.

      The protein encoded by TXN gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for TXN (provided by RefSeq, Oct 2011).

      Mouse anti Human thioredoxin 1 antibody detects a band of 12 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti thioredoxin 1 detects a band of approximately 12 kDa in HeLa cell lysates
      Anti thioredoxin 1 detects a band of approximately 12 kDa in HeLa cell lysates
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
    • Buffer Solution
      HEPES buffered saline
      HEPES buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      E. coli-derived recombinant human thioredoxin 1
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg HeLa lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      cell proliferation
      signal transduction
      cell-cell signaling
      transcription
      protein binding
      electron transport chain
      cytosol
      extracellular region
      nucleobase, nucleoside and nucleotide interconversion
      nucleoplasm
      regulation of protein import into nucleus, translocation
      response to radiation
      cellular component movement
      transport
      positive regulation of DNA binding
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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