TGN46 Antibody

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TGN46 Antibody gallery image 1

Published customer image:
Rabbit anti Human TGN46 antibody used for the localisation of the trans golgi network in HeLa cells by immunofluorescence.
Image caption:
Nuclear import of HPV16 vDNA is blocked in interphase cells. (A) Aphidicolin-treated or untreated HeLa cells were infected with HPV16-GFP. At 16 h p.i., cells were fixed and immunostained with the L1-7 antibody detecting endosomal, conformationally altered virions. Depicted are confocal sections of untreated (left) or aphidicolin-treated (right) cells. (B) As in (A) with quantification of the three-dimensional distance of L1-7 signals to the nuclear border (in µm) ± SD. (C) As in (A) with quantification of the number of discernible L1-7 spots/cell. (D) As in (A) with quantification of the fluorescent intensity (in arbitrary units, AU) of individual L1-7 spots. (E) HeLa H2B-mCherry cells were infected with BrdU-HPV16. Cells were immunostained for BrdU to detect the vDNA after fixation at 24 h p.i.. Depicted are confocal sections of aphidicolin-treated (interphase, right) or untreated (left) cells. Mostly, the vDNA localized intranuclearly as discrete spots in untreated cells (arrowheads) or exclusively perinuclearly in aphidicolin-treated cells. (F) As in (E) with quantification of intranuclear BrdU spots/cell. The number of intranuclear spots is given relative to the total number of cellular spots. (G) As in (E) with quantification of perinuclear BrdU spot/cell as in (F). (H) As in (E) with quantification of the signal intensities of individual perinuclear BrdU spots relative to untreated infected cells. (I) HeLa cells were treated with or without aphidicolin for 16 h prior to infection. 20 h after infection with EdU-HPV16 (green), cells were fixed and immunostained for TGN46 (red) and LAMP1 (light blue). The nucleus was stained by Hoechst (dark blue). Depicted are single confocal sections. The cytosolic EdU-HPV16 signal is depicted after substraction of the EdU-HPV16 signals colocalizing with LAMP1, TGN46 and the nucleus. (J) As (I) with quantification of signal colocalization of EdU-HPV16 with LAMP1 or TGN46. Cytosolic EdU-HPV16 amounts were defined as signals that did not colocalize with LAMP1, TGN46, or the nucleus (see below). (K) As in (I) with quantification of signal colocalization of EdU-HPV16 with the nuclear stain (Hoechst). Statistical significance was determined by a two-tailed, independent t-test; P-values: * <0.05; *** <0.001. All scale bars: 10 µm.

From: Aydin I, Weber S, Snijder B, Samperio Ventayol P, Kühbacher A, Becker M, et al. (2014) Large Scale RNAi Reveals the Requirement of Nuclear Envelope Breakdown for Nuclear Import of Human Papillomaviruses. PLoS Pathog 10(5): e1004162.

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TGN46 Antibody gallery image 2

Published customer image:
Rabbit anti Human TGN46 antibody used to identify the tans-golgi network in transfected HeLa cells by immunofluorescence.
Image caption:
HSV-1 gN is an ER resident that requires the hydrophobic core of gM for transport to the TGN. To follow their subcellular distribution, gN–EYFP or HA–gM were transiently expressed either alone or combining gN–EYFP with either full-length (HA–gM) or N- and C-terminal truncation mutants of gM (HA–gM 133–473, HA–gM 1–433, HA–gM 1–422, HA–gM 1–361, HA–gM 1–342) in HeLa cells for 20 h. Anti-HA antibodies were used to detect the localization of gM and gM truncations, followed by secondary reagents. gN–EYFP was visualized directly. Antibodies recognizing the marker proteins Calreticulin (CRT) and TGN46 were used to visualize the ER and the TGN, respectively. Nuclei were visualized by DAPI staining. The scale bars correspond to 10 μm.

From: Striebinger, H.; Funk, C.; Raschbichler, V.; Bailer, S.M.
Subcellular Trafficking and Functional Relationship of the HSV-1 Glycoproteins N and M.
Viruses 2016, 8, 83.

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TGN46 Antibody gallery image 3

Published customer image:
Rabbit anti Human TGN46 antibody used for the localisation of the trans golgi network in HeLa cells by immunofluorescence.
Image caption:
Nuclear import of HPV16 vDNA is blocked in interphase cells. (A) Aphidicolin-treated or untreated HeLa cells were infected with HPV16-GFP. At 16 h p.i., cells were fixed and immunostained with the L1-7 antibody detecting endosomal, conformationally altered virions. Depicted are confocal sections of untreated (left) or aphidicolin-treated (right) cells. (B) As in (A) with quantification of the three-dimensional distance of L1-7 signals to the nuclear border (in μm) ± SD. (C) As in (A) with quantification of the number of discernible L1-7 spots/cell. (D) As in (A) with quantification of the fluorescent intensity (in arbitrary units, AU) of individual L1-7 spots. (E) HeLa H2B-mCherry cells were infected with BrdU-HPV16. Cells were immunostained for BrdU to detect the vDNA after fixation at 24 h p.i.. Depicted are confocal sections of aphidicolin-treated (interphase, right) or untreated (left) cells. Mostly, the vDNA localized intranuclearly as discrete spots in untreated cells (arrowheads) or exclusively perinuclearly in aphidicolin-treated cells. (F) As in (E) with quantification of intranuclear BrdU spots/cell. The number of intranuclear spots is given relative to the total number of cellular spots. (G) As in (E) with quantification of perinuclear BrdU spot/cell as in (F). (H) As in (E) with quantification of the signal intensities of individual perinuclear BrdU spots relative to untreated infected cells. (I) HeLa cells were treated with or without aphidicolin for 16 h prior to infection. 20 h after infection with EdU-HPV16 (green), cells were fixed and immunostained for TGN46 (red) and LAMP1 (light blue). The nucleus was stained by Hoechst (dark blue). Depicted are single confocal sections. The cytosolic EdU-HPV16 signal is depicted after substraction of the EdU-HPV16 signals colocalizing with LAMP1, TGN46 and the nucleus. (J) As (I) with quantification of signal colocalization of EdU-HPV16 with LAMP1 or TGN46. Cytosolic EdU-HPV16 amounts were defined as signals that did not colocalize with LAMP1, TGN46, or the nucleus (see below). (K) As in (I) with quantification of signal colocalization of EdU-HPV16 with the nuclear stain (Hoechst). Statistical significance was determined by a two-tailed, independent t-test; P-values: * <0.05; *** <0.001. All scale bars: 10 μm.

From: Aydin I, Weber S, Snijder B, Samperio Ventayol P, Kühbacher A, Becker M, et al. (2014) Large Scale RNAi Reveals the Requirement of Nuclear Envelope Breakdown for Nuclear Import of Human Papillomaviruses. PLoS Pathog 10(5): e1004162.

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  • Rabbit anti Human TGN46
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1586IF*, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP1586
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human TGN46 antibody recognizes Trans-Golgi network integral membrane protein 2, also known as TGN46, TGN38 homolog or Trans-Golgi network protein TGN51. TGN46 is a 480 amino acid ~110kDa single pass type I transmembrane glycoprotein associated with the trans golgi network membrane. TGN46 has been reported as being the best available marker for human trans-Golgi network.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Primateyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human TGN46 were raised by repeated immunisation of rabbits with highly purified recombinant human TGN46.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Recombinant human TGN46.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/000
      Immunofluorescence(1)1/501/100
      (1)
      Fixation of tissues with 3% paraformaldehyde or methanol is recommended prior to using this antibody on immunofluorescence.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TGN46 Antibody Formats

    Formats Applications Sizes available
    TGN46 Antibody : Serum IF*, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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