TGF Alpha Antibody

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Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human TGF alpha (AHP284G)

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  • Rabbit anti Human TGF Alpha:Biotin
  • Rabbit anti Human TGF Alpha
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP284GE, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP284G
    AHP284BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP284B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti human TGFα recognizes tranforming growth factor alpha. TGFα is a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS).

      TGFα is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGFα is mitogenic for multipotent neural progenitors and is involved in astrogliosis (Junier 2000).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
    • Preparation
    • Antiserum Preparation
      Antisera to human TGFα were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml after reconstitution
      IgG concentration 1.0 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of cell division
      integral to membrane
      signal transducer activity
      activation of MAPK activity
      MAP kinase kinase activity
      positive regulation of epidermal growth factor receptor activity
      cell proliferation
      growth factor activity
      extracellular space
      positive regulation of epithelial cell proliferation
      epidermal growth factor receptor binding
      cell surface
      plasma membrane
      positive regulation of mitosis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.150.30ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays2.53.7ug/ml
      Western Blotting0.1ug/ml0.2ug/ml
      Immunohistology - Paraffin(1)0.5ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human TGFα antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human TGFα antibody (AHP284G) as the capture reagent and recombinant human TGFα (PHP037) as the standard.
    • ELISA
      This purifed human TGFα antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human TGFα antibody (AHP284B) as the detection reagent and recombinant human TGFα (PHP037) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Rabbit anti human TGF&alpha may be used in Western Blotting applications under either reducing or non-reducing condtions with recombinant human TGFα (PHP037) as the positive control.
    • Western Blotting
      Rabbit anti human TGFα may used in Western Blotting application under either reducing or non-reducing conditions with recombinant human TGFα (PHP037) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional TGF Alpha Antibody Formats

    Formats Applications Sizes available
    TGF Alpha Antibody : Biotin E, WB 50 µg
    TGF Alpha Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR121B
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      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

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