Mouse anti Human TdT antibody panel, clones HT-1, HT-4, Ht-8 and HT-9 is a mixture of 4 monoclonal antibodies specific for human terminal deoxynucleotidyl transferase (TdT). TdT is a DNA polymerase responsible for the catalysis of non-reversible addition of deoxynucleotides to the 3' end hydroxy groups of DNA. TdT levels are enhanced in all forms of acute lymphoblastic leukaemia (ALL) and in a significant number of chronic lymphoblastic leukaemia cases (CML). TdT is not expressed in the majority of myeloid leukaemias, non-Hodgkins lymphomas or mature lymphoid leukaemias.
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.