TdT | HT-1, HT-4, HT-8, HT-9

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  • Mouse anti Human TdT:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody Panel
  • Clone
    HT-1, HT-4, HT-8, HT-9
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT0012F *datasheet pdfdatasheet pdf25 µg
    OBT0012
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human TdT antibody panel, clones HT-1, HT-4, Ht-8 and HT-9 is a mixture of 4 monoclonal antibodies specific for human terminal deoxynucleotidyl transferase (TdT). TdT is a DNA polymerase responsible for the catalysis of non-reversible addition of deoxynucleotides to the 3' end hydroxy groups of DNA. TdT levels are enhanced in all forms of acute lymphoblastic leukaemia (ALL) and in a significant number of chronic lymphoblastic leukaemia cases (CML). TdT is not expressed in the majority of myeloid leukaemias, non-Hodgkins lymphomas or mature lymphoid leukaemias.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50.0 ug/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date.
    • GO Terms
      DNA replication
      DNA modification
      DNA-directed DNA polymerase activity
      DNA nucleotidylexotransferase activity
      metal ion binding
      nucleus
      DNA binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TdT Formats

    Formats Clone Applications Sizes available
    TdT : FITC HT-1, HT-4, HT-8, HT-9 F * 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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