TCF4 Antibody (PrecisionAbTM Antibody) | AB01/4A12-6-6

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TCF4 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/4A12-6-6 gallery image 1

Western blot analysis of whole cell lysates probed with TCF4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 118 second exposure. Arrow points to TCF4 (molecular weight 55 kDa).

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  • TCF4 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/4A12-6-6 thumbnail image 1
  • TCF4 Antibody with Control Lysate
  • Mouse anti TCF4
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    TCF4 antibody recognizes transcription factor 4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00631WBdatasheet pdfdatasheet pdf100 µl
      VMA00631
      VMA00631KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00631KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human TCF4 antibody recognizes TCF4, also known as transcription factor 4, SL3-3 enhancer factor 2, class B basic helix-loop-helix protein 19 or immunoglobulin transcription factor 2.

        TCF4 encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is broadly expressed, and may play an important role in nervous system development. Defects in TCF4 are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described (provided by RefSeq, Aug 2011).

        Mouse anti Human TCF4 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
        Mouse anti TCF4 antibody detects a band of approximately 55 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E. coli-derived recombinant human TCF4 (aa 1-365)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        armadillo repeat domain binding
        negative regulation of canonical Wnt receptor signaling pathway
        transcription factor complex
        TFIIB-class transcription factor binding
        TFIIB-class binding transcription factor activity
        beta-catenin binding
        RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
        E-box binding
        orbitofrontal cortex development
        transcription initiation from RNA polymerase II promoter
        cerebral cortex development
        protein heterodimerization activity
        protein-DNA complex assembly
        RNA polymerase II core promoter proximal region sequence-specific DNA binding
        specific transcriptional repressor activity
        regulation of smooth muscle cell proliferation
        transcription initiation factor activity
        sequence-specific DNA binding RNA polymerase recruiting transcription factor activity
        protein C-terminus binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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