Tamm Horsfall Glycoprotein Antibody

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  • Sheep anti Human Tamm Horsfall Glycoprotein
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    8595-0054C, Edatasheet pdfdatasheet pdf1 ml
    8595-0054
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Human Tamm Horsfall Glycoprotein antibody is specific for human Tamm Horsfall Protein (THP), also known as uromodulin (Pennica et al. 1987). THP was originally isolated from normal human urine (Tamm et al. 1952). THP is a 616 amino acid ~85kDa glycoprotein synthesized by the kidney and is the most abundant urinary protein in healthy individuals (Pennica et al. 1987). THP forms the matrix of all urinary casts either alone (hyaline casts) or in association with cellular fragments and filtered proteins (granular casts), casts are indicative of a number of pathological states including glomerulonephritis and vasculitis (Chambers et al. 1986).

      The specificity of sheep anti human Tamm Horsfall glycoprotein antibody has been confirmed by double diffusion against tamm horsfall glycoprotein and an independent known antibody against THP.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      IgG fraction - liquid
    • Reconstitution
    • Preparation
      IgG fraction prepared by ammonium sulphate fractionation
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.1%EACA
      0.01%Benzamidine
      1 mMEDTA
    • Immunogen
      Purified human uromucodulin prepared from urine.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      negative regulation of cell proliferation
      calcium ion binding
      cellular defense response
      apical plasma membrane
      extrinsic to membrane
      extracellular region
      anchored to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/40,000
      Immunohistology - Frozen1/501/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Tamm Horsfall Glycoprotein Antibody Formats

    Formats Applications Sizes available
    Tamm Horsfall Glycoprotein Antibody : Purified C, E 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Immunohistology - Paraffin

              References

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