TAK1 Antibody

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TAK1 Antibody gallery image 1

Western blotting analysis of transfected HEK293 lysate transiently expressing human TAK1 protein with a DYKDDDK tag, probed with Goat anti Human TAK1 antibody (AHP2077) in Lane A, mock transfected cells probed with the same antibody in lane B and transfected cells probed with an anti DYKDDDK tag antibody in lane C.

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  • Goat anti Human TAK1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2077E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2077
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Goat anti Human TAK1 antibodyrecognizes human Mitogen-activated protein kinase kinase kinase 7, also known as TAK1, Transforming growth factor-beta-activated kinase 1 or MAP3K7. TAK1 is a 606 amino acid ~67 kDa serine threonine kinase, an essential component of the MAP kinase signal transduction pathway.

      Four potential isoforms of TAK1, caused by alternative splicing have been identified. Goat anti Human TAK1 antibody is expected to bind the canonical isoform 1B, expressed mainly in the brain, kidney and small intestine. Goat anti Human TAK1 antibody is also expected to recognize isoform 1a, expressed mainly in ovary, skeletal muscle, spleen and blood mononuclear cells. The antibody is not however expected to recognize the alternatively spliced isoforms 1c and 1d, both of which lack the c-terminal portion of TAK1 including the region covering the immunogen sequence used to generate this Goat anti Human TAK1 antibody (aa 552-564. UniProt : O43318).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1000
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      n transfected HEK293 transiently expressing full-length Human MAP3K7 (myc and DYKDDDDK tagged), a band of approx. 75 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2μg/ml.
    • Instructions For Use

    Additional TAK1 Antibody Formats

    Formats Applications Sizes available
    TAK1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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