TAB2 Antibody (PrecisionAbTM Antibody)

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TAB2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with TAB2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to TAB2 (molecular weight 88 kDa)

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  • TAB2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • TAB2 Antibody with Control Lysate
  • Mouse anti TAB2
  • TAB2 Antibody with Control Lysate
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    TAB2 antibody recognizes TGF-beta-activated kinase 1/MAP3K7-binding protein 2, also known as TAK1-binding protein 2 or mitogen-activated protein kinase kinase kinase 7-interacting protein 2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00296WBdatasheet pdfdatasheet pdf100 µl
      VMA00296
      VMA00296KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00296K
      VMA00296KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00296KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human TAB2 antibody recognizes the TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, also known as TAK1-binding protein 2, mitogen-activated protein kinase and kinase kinase 7-interacting protein 2.

        Encoded by the TAB2 gene, TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 is an activator of MAP3K7/TAK1, which is required for for the IL-1 induced activation of nuclear factor kappaB and MAPK8/JNK. This protein forms a kinase complex with TRAF6, MAP3K7 and TAB1, thus serves as an adaptor linking MAP3K7 and TRAF6. This protein, TAB1, and MAP3K7 also participate in the signal transduction induced by TNFSF11/RANKl through the activation of the receptor activator of NF-kappB (TNFRSF11A/RANK), which may regulate the development and function of osteoclasts (provided by RefSeq, Jul 2008).

        Mouse anti Human TAB2 antibody detects a band of 88 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti TAB2 detects a band of approximately 88 kDa in HEK293 cell lysates
        Anti TAB2 detects a band of approximately 88 kDa in HEK293 cell lysates
        Anti TAB2 detects a band of approximately 88 kDa in HEK293 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified recombinant fragment of human TAB2 expressed in E. coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        toll-like receptor 3 signaling pathway
        positive regulation of I-kappaB kinase/NF-kappaB cascade
        zinc ion binding
        heart development
        positive regulation of NF-kappaB transcription factor activity
        innate immune response
        MyD88-dependent toll-like receptor signaling pathway
        T cell receptor signaling pathway
        I-kappaB kinase/NF-kappaB cascade
        toll-like receptor 4 signaling pathway
        JNK cascade
        toll-like receptor 1 signaling pathway
        endosome membrane
        K63-linked polyubiquitin binding
        stress-activated MAPK cascade
        cytosol
        MyD88-independent toll-like receptor signaling pathway
        toll-like receptor 2 signaling pathway
        activation of MAPK activity
        plasma membrane
        Toll signaling pathway
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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