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Synphilin 1 Antibody

Synphilin 1 Antibody gallery image 1

Published customer image:
Goat anti Human synphilin 1 antibody used for immunofluorescence
Image caption:
Synphilin-1 interaction with lipid rafts in membranes of mouse primary neurons. A: Pictures obtained by confocal microscopy to determine co-localization of inclusions formed by mouse synphilin-1 with lipid rafts in membranes of mouse primary neuronal cultures. Shown are representative pictures taken at the plane of the neurites (left panels) or of the neuronal cell body (right panels). Synphilin-1 was detected using a goat anti-synphilin 1 as primary antibody and donkey anti-goat IgG (H+L) coupled to Alexa Fluor 568 as secondary antibody. The cholera toxin subunit B served as a marker for lipid rafts. DAPI was used to stain nuclei. The white bar in the upper panel corresponds to a size of 10 µm. B: Interaction with lipid rafts of endogenous SYWT and a-Syn in membranes of mouse primary neuronal cultures. Lipid raft fractions (boxed areas) were identified using the mammalian lipid raft marker flotillin-1. The open arrowhead indicates the position of the 100 kDa molecular weight marker.

From: :Büttner S, Delay C, Franssens V, Bammens T, Ruli D, et al. (2010) Synphilin-1 Enhances a-Synuclein Aggregation in Yeast and Contributes to Cellular Stress and Cell Death in a Sir2-Dependent Manner. PLoS ONE 5(10): e13700.

  • Synphilin 1 Antibody thumbnail image 1
  • Goat anti Human Synphilin 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP593E, IF, P, WBdatasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • AHP593 recognises synphilin-1, a cytoplasmic protein which associates with alpha-synuclein in vivo. Alpha-Synuclein is a major component of lewy bodies in patients with sporadic Parkinson's disease (PD) and mutations of alpha-synuclein are involved in familial forms of PD. Reports suggest that synphilin-1 may play a role in lewy body formation in association with alpha synuclein.
    • Intended Use
    • Target Species
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Synphilin-1 were raised by repeated immunisation of goat with highly purified antigen.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/400
      Western Blotting1/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human Brain
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Synphilin 1 Antibody Formats

    Formats Applications Sizes available
    Synphilin 1 Antibody : Serum E, IF, P, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human Brain
                • Product Images

                • Synphilin 1 Antibody thumbnail image 1


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