Syk Antibody

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  • Goat anti Syk
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1066E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1066
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Syk antibody detects an epitope within the internal region of SYK, a ~70 kDa non-receptor protein tyrosine kinase whose role differs in haematopoietic and non haematopoietic stem cells. In haematopoietic cells SYK is involved in the proximal signalling downstream of activated immunoreceptors (BCR, TCR, Fc receptors), thereby orchestrating a complex series of cellular responses such as cell proliferation, differentiation, survival and phagocytosis.

      In epithelial cells SYK appears to function as a negative regulator of related events. Furthermore, SYK has been shown to promote expression or secretion of inflammation-related molecules in lung epithelial cells.

      SYK appears to be required for vascular integrity during development, thus, manipulation of SYK function in endothelial cells could potentially modulate tumour-associated angiogenesis. SYK also has been shown to interact with Epstein-Barr virus LMP2A.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      DogExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to SYK were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide (NaN3)
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide with sequence C-KYLQQNRHVKDKN from the internal region of the SYK sequence.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cytosol
      integrin binding
      platelet activation
      T cell receptor complex
      non-membrane spanning protein tyrosine kinase activity
      interspecies interaction between organisms
      integrin-mediated signaling pathway
      protein complex assembly
      leukocyte cell-cell adhesion
      ATP binding
      neutrophil chemotaxis
      cell proliferation
      organ morphogenesis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/16000
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1066 detects a band of approximately 70kDa in human bone marrow, lymph node and spleen lysates.
    • Instructions For Use

    Additional Syk Antibody Formats

    Formats Applications Sizes available
    Syk Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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