Superoxide Dismutase (MN) Antibody | 2A1

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Superoxide Dismutase (MN) Antibody | 2A1 gallery image 1

Mouse anti superoxide dismutase (Mn) recognizes a band of approximately 23 kDa in HepG2 cell lysate (lane 1), HeLa cell lysate (lane 2) mouse brain tissue lysate (lane 3) and rat brain tissue lysate (lane 4)

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  • Mouse anti Superoxide Dismutase (MN)
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  • Product Type
    Monoclonal Antibody
  • Clone
    2A1
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6027WBdatasheet pdfdatasheet pdf50 µg
    MCA6027
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti superoxidase dismutase (Mn), clone 2A1, recognizes superoxide dismutase 2 (Mn) also known as superoxide dismutase 2 (SOD2). SOD2 is an anti oxidant enzyme involved in the body's defense against reactive oxygen species. Superoxide dismutases destroy damaging free radicals that are produced within cells and are toxic to biological systems.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.03% Sodium Azide (NaN3)
      0.01% Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Recombinant human protein purified from E.coli
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      HEPES buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      superoxide dismutase activity
      negative regulation of neuron apoptosis
      mitochondrial matrix
      release of cytochrome c from mitochondria
      regulation of transcription from RNA polymerase II promoter
      manganese ion binding
      protein binding
      protein homotetramerization
      age-dependent response to reactive oxygen species
      removal of superoxide radicals
      vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
      oxygen homeostasis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse anti superoxide dismutase (Mn) recognizes a band of approximately 23 kDa in HeLa and HepG2 cell lysates and mouse and rat brain tissue lysates
    • Instructions For Use

    Additional Superoxide Dismutase (MN) Antibody Formats

    Formats Clone Applications Sizes available
    Superoxide Dismutase (MN) Antibody : Purified 2A1 WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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