Sulfatase 2 antibody | 2B4
Mouse anti Human Sulfatase 2 (C-Terminal)
- Product Type
- Monoclonal Antibody
- Clone
- 2B4
- Isotype
- IgG1
- Specificity
- Sulfatase 2
- Region
- (C-TERMINAL)
Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Sulf-2 is linked with several cancers, including breast, hepatocellular, pancreatic and non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50 kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75 kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide.
- Carrier Free
- Yes
- Immunogen
- Recombinant human Sulf-2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Paraffin 1 | |||
Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
- Western Blotting
- Mouse anti Human sulfatase 2 antibody, clone 2B4 detects a major band of ~50kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.
References for Sulfatase 2 antibody
-
Lemjabbar-Alaoui, H. et al. (2010) Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis.
Oncogene. 29 (5): 635-46. -
Lui NS et al. (2012) SULF2 expression by immunohistochemistry and overall survival in oesophageal cancer: a cohort study.
BMJ Open. 2 (6): pii: e001624. -
Phillips, J.J. et al. (2012) Heparan sulfate sulfatase SULF2 regulates PDGFRα signaling and growth in human and mouse malignant glioma.
J Clin Invest. 122 (3): 911-22. -
Alhasan, S.F. et al. (2016) Sulfatase-2: a prognostic biomarker and candidate therapeutic target in patients with pancreatic ductal adenocarcinoma.
Br J Cancer. 115 (7): 797-804.
- Synonyms
- SULF-2
- RRID
- AB_10707932
- UniProt
- Q8IWU5
- Q8CFG0
- Entrez Gene
- Sulf2
- SULF2
- GO Terms
- GO:0005509 calcium ion binding
- GO:0004065 arylsulfatase activity
- GO:0005783 endoplasmic reticulum
- GO:0005795 Golgi stack
- GO:0009986 cell surface
- GO:0005615 extracellular space
- GO:0030201 heparan sulfate proteoglycan metabolic process
MCA5692GA
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