Substance P Antibody

//www.bio-rad-antibodies.com/human-substance-p-antibody-8450-0004.html
Substance P Antibody gallery image 1

Staining of paraffin embedded human appendix with Rabbit anti Human Substance P (8450-0004)

Enlarge
Substance P Antibody gallery image 2

Published customer image:
Rabbit anti Human Substance P antibody used to demonstrate substance P staining in keratocytes by immunofluorescence.
Image caption:
Substance P and neurokinin A expression in keratocytes of cornea tissue sections and in cultured primary keratocytes. (A-B) Substance P (A) and neurokinin A (B) EIA of central and peripheral cultured keratocytes. 250,000 cells were grown for 24h in 6 well plates in DMEM/F12 supplemented with either 2% or 0% FBS. Supernatant was collected, cells were lysed and subjected to EIA. (A) SP levels were significantly higher in supernatants of central keratocytes grown in medium supplemented with 2% FBS than in peripheral keratocytes grown in medium supplemented with 2% FBS (***p<0.001). SP secretion was significantly higher in central keratocytes grown in 2% FBS than central keratocytes grown in 0% FBS (***p<0.001). There were no significant differences in SP levels in lysates of keratocytes. (B) NKA secretion was significantly higher in central keratocytes grown in 2% FBS than in central keratocytes grown in 0% FBS (*p<0.05). (C) Keratocytes in sections (left panel), as well as cultured central (middle panel) and peripheral (right panel) keratocytes, were labeled with an antibody targeting SP (top row; TRITC; red) and double stained with DAPI to visualize the nuclei (blue). Sections and cultured cells were also labeled with an antibody targeting full-length NK-1R (bottom row; Alexa Fluor 488; green) and double stained with DAPI. Both the keratocytes in tissue sections and the cultured keratocytes expressed SP and a full-length version of NK-1R. (D) Keratocytes in sections (left panel), as well as cultured central (middle panel) and peripheral (right panel) keratocytes, were labeled with an antibody targeting NKA (top row; TRITC; red) and NK-2R (bottom row; TRITC; red) and double stained with DAPI. Both the keratocytes in tissue sections and the cultured keratocytes expressed NKA and NK-2R. (E-F) 250,000 central keratocytes or peripheral keratocytes grown in DMEM/F12 supplemented with either 2% or 0% FBS were subjected to western blot analysis for NK-1R (46 kDa) and NK-2R (48 kDa) expression. (E) Expression of NK-1R was significantly higher in central keratocytes grown in 2% FBS than in peripheral keratocytes grown in same conditions (***p<0.001). Expression of NK-1R was also significantly higher in central keratocytes grown in 2% FBS than in central keratocytes grown in 0% FBS (***p<0.001). (F) Expression of NK-2R was significantly higher in central keratocytes grown in 0% FBS than in peripheral keratocytes grown in same conditions (***p<0.001). NK-2R expression was significantly higher in central keratocytes grown in 0% FBS than in central keratocytes grown in 2% (***p<0.001). n.s. (non significant; p>0.05). β-actin (42 kDa) served as a loading control. Values are means ± SD.

From: Sloniecka M, Le Roux S, Boman P, Byström B, Zhou Q, Danielson P (2015) Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ.
PLoS ONE 10(7): e0134157.

Enlarge
Substance P Antibody gallery image 3

Published customer image:
Rabbit anti Human Substance P antibody used for the detection of substance P in human Achilles tendon cells by immunofluorescence.
Image caption:
Human Achilles tendon cells in culture immunopositive for SP and vimentin. (a) Cells stained with DAPI (bluish), to mark nuclei, and immunolabeled for vimentin (red; TRITC-staining). (b) Same cells stained with DAPI and immunolabeled for SP (green; FITC-staining). Clear SP-reactions are seen close to the nuclei (arrows). (c) Digitally merged image of a and b; SP appearing in yellow colour.

From: Backman LJ, Fong G, Andersson G, Scott A, Danielson P (2011) Substance P Is a Mechanoresponsive, Autocrine Regulator of Human Tenocyte Proliferation.
PLoS ONE 6(11): e27209.

Enlarge
Substance P Antibody gallery image 4

Published customer image:
Rabbit anti Human Substance P antibody used for the detection of substance P in human Achilles tendon cells by immunofluorescence.
Image caption:
Human Achilles tendon cells in culture immunopositive for SP and vimentin. (a) Cells stained with DAPI (bluish), to mark nuclei, and immunolabeled for vimentin (red; TRITC-staining). (b) Same cells stained with DAPI and immunolabeled for SP (green; FITC-staining). Clear SP-reactions are seen close to the nuclei (arrows). (c) Digitally merged image of a and b; SP appearing in yellow colour.

From: Backman LJ, Fong G, Andersson G, Scott A, Danielson P (2011) Substance P Is a Mechanoresponsive, Autocrine Regulator of Human Tenocyte Proliferation.
PLoS ONE 6(11): e27209.

Enlarge
  • Substance P Antibody thumbnail image 1
  • Substance P Antibody thumbnail image 2
  • Substance P Antibody thumbnail image 3
  • Substance P Antibody thumbnail image 4
  • Rabbit anti Human Substance P
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    8450-0004C, IF, P *datasheet pdfdatasheet pdf0.5 ml
    8450-0004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Substance P antibody recognizes human Protachykinin-1, also known as PPT. Protachykinin-1 is a 129 amino acid ~15kDa tachychinin involved in the excitation of neurons. Protachykinin-1 can be cleaved into a number of chains including substance P, neurokinin A, neuropeptide K, neuropeptide γ and c-terminal flankning peptide. Rabbit anti Human Substance P antibody recognizes an epitope withing the substance P region of the molecule. There are potentially four isoforms of Protachykinin-1 (α - δ) formed by alternative splicing, all containing the substance P peptide region (UniProt: P20366).

      Rabbit anti Human Substance P antibody stains substance P secreting cells of the external median eminance of the hypothalamus and specific cells of the GI tract. Rabbit anti Human Substance P antibody also stains neuronal structures.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chickenyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Synthetic substance P conjugated to Keyhole limpet hemocyanin.
    • Purity
    • Approx. Protein Concentrations
      5.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen1/501/200
      Immunohistology - Paraffin(1)1/501/200
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Appendix
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Substance P Antibody Formats

    Formats Applications Sizes available
    Substance P Antibody : Purified C, IF, P * 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Appendix

              References

              Write your review

              You may also be interested in...