STIM1 Antibody | 5A2

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STIM1 Antibody | 5A2 gallery image 1

Immunofluorescence showing STIM1 in HeLa cells using Mouse anti Human STIM1:Azide Free (MCA3475Z)

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STIM1 Antibody | 5A2 gallery image 2

Staining of paraffin embedded human diffuse large B-cell lymphoma with Mouse anti Human STIM1:Azide Free (MCA3475Z)

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STIM1 Antibody | 5A2 gallery image 3

Western blot analysis of STIM1 expression in K-562 using Mouse anti Human STIM1:Azide Free (MCA3475Z)

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STIM1 Antibody | 5A2 gallery image 4

Western blot analysis of STIM1 expression in HeLa using Mouse anti Human STIM1:Azide Free (MCA3475Z)

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  • Mouse anti Human STIM1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5A2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3475ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3475Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human STIM1 antibody, clone 5A2 recognizes human Stromal interaction molecule 1, also known as STIM1. STIM1 is a 663 amino acid ~80 kDa single pass type I transmembrane glycoprotein involved in replenishment of depleted intracellular Ca2+.

      Mutations in the STIM1 gene can lead to development of Immunodeficiency 10 (IMD10) characterized by recurrent infections and impaired T-cell responses (Fuchs et al. 2012) or of Myopathy, tubular aggregate, 1 (TAM1), a rare developmental myopathy with regular arrays of membrane tubules on muscle (Böhm et al. 2013).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 24-686 of human STIM1
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      activation of store-operated calcium channel activity
      integral to plasma membrane
      platelet activation
      detection of calcium ion
      calcium ion transport
      calcium ion binding
      microtubule
      microtubule plus-end binding
      integral to endoplasmic reticulum membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional STIM1 Antibody Formats

    Formats Clone Applications Sizes available
    STIM1 Antibody : Preservative Free 5A2 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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