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Stem Cell Factor

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  • Recombinant Human Stem Cell Factor
  • Recombinant Human Stem Cell Factor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP067E, FN, WBdatasheet pdfdatasheet pdf10 µg
    PHP067
    PHP067AE, FN, WBdatasheet pdfdatasheet pdf2 µg
    PHP067A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Stem cell factor (SCF) is a haematopoietic growth factor acting during early stages of haemopoiesis. SCF stimulates proliferation of myeloid, erythroid and lymphoid progenitors in bone marrow cultures and appears to act synergistically with the colony stimulating factors.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Pack Size: 2 µgReconstitute with 20 ul distilled water
      Pack Size: 10 µgReconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 2 µgReconstitute with 20 ul distilled water
      Pack Size: 10 µgReconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present.
      None present.
    • Activity
      5 x 105 U/mg as determined by dose-dependent stimulation of proliferation on human TF-1 cells.
    • Purity
      >98% by SDS PAGE and HPLC analysis
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      Pack Size: 2 µg0.1 mg/ml
      Pack Size: 10 µgTotal protein concentration 0.1mg/ml after reconstitution
      Pack Size: 2 µg0.1 mg/ml
      Pack Size: 10 µgTotal protein concentration 0.1mg/ml after reconstitution
    • Protein Molecular Weight
      18.4kD (165 Amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1ng/ug
    • Test Principle
    • Buffer Solution
      Pack Size: 10 µg10mM Acetic acid
      Pack Size: 10 µg10mM Acetic acid
    • Storage
      Pack Size: 2 µgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 10 µgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 2 µgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 10 µgPrior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution
      3 months from date of reconstitution
    • GO Terms
      stem cell factor receptor binding
      plasma membrane
      growth factor activity
      male gonad development
      integral to membrane
      positive regulation of DNA replication
      identical protein binding
      signal transduction
      cytoskeleton
      cell adhesion
      hemopoiesis
      cytoplasm
      cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      0.2Pack Size: 10 µg
      0.2ng/mlPack Size: 2 µg
      20.0ng/mlPack Size: 10 µg
      20ng/mlPack Size: 2 µg
      Western Blotting1.53.0ng/lane
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      0.2Pack Size: 10 µg
      0.2ng/mlPack Size: 2 µg
      20.0ng/mlPack Size: 10 µg
      20ng/mlPack Size: 2 µg
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      PHP067 may be used as a standard for ELISA applications with AHP1210
    • ELISA
      Recombinant human SCF may be used as a standard for ELISA applications with our human SCF polyclonal antibody (AHP1210).
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      PHP067 may be used as a positive control for Western blotting applications with AHP1210
    • Western Blotting
      Recombinant human SCF may be used as the positive control for Western Blotting applications with our human SCF polyclonal antibody (AHP1210).
    • Instructions For Use
    • Instructions For Use

    Additional Stem Cell Factor Formats

    Formats Applications Sizes available
    Stem Cell Factor : Rec. Protein E, FN, WB 2 µg | 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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