Stem Cell Factor Antibody

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Stem Cell Factor Antibody gallery image 1

Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (AHP1210) following enzyme mediated antigen retrieval using proteinase K

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Stem Cell Factor Antibody gallery image 2

Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (AHP1210) following enzyme mediated antigen retrieval using proteinase K

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Stem Cell Factor Antibody gallery image 3

Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (AHP1210) following heat mediated antigen retrieval using EDTA buffer (pH9.0)

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Stem Cell Factor Antibody gallery image 4

Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (AHP1210) following enzyme mediated antigen retrieval using proteinase K

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  • Goat anti Human Stem Cell Factor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1210E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1210
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human Stem Cell Factor antibody recognizes human stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor.

      Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The association between SCF and c-Kit is also involved with the progression and invasiveness of tumours, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays0.0080.012ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
      Successful heat mediated antigen retrieval has also been accomplished using Tris/EDTA buffer (pH9.0).

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      AHP1210 may be used in an indirect ELISA with PHP067 as the standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1210 may be used for Western blotting with PHP067 as the positive control.
    • Instructions For Use

    Additional Stem Cell Factor Antibody Formats

    Formats Applications Sizes available
    Stem Cell Factor Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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