Rabbit anti Stella antibody recognizes human Stella, also known as Developmental pluripotency-associated protein 3 (DPP3).
Stella is a marker for pluripotency in embryonic stem (ES) cells as its expression is observed in primordial germ cells of both sexes and germ cell tumors but not in normal somatic tissues. However, in ES cell colonies, heterogeneous expression of Stella was seen in high throughput in situ hybridization assays, indicating that higher levels of complexity exist in otherwise thought to be undifferentiated ES cells. At least two distinct isoforms of Stella are known to exist.
Stella is highly expressed in testicular germ cell tumours. It is also expressed in embryonic stem and carcinoma cells. Low expression is observed in tissues of the testis, ovary and thymus.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antisera to human Stella were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
0.02% Sodium Azide (NaN3)
Synthetic peptide sequence corresponding to a 13 amino acid sequence from near the carboxy terminus of Human Stella
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1798 detects a band of approximately 18 kDa in 293 cell lysate