STAT6 (pTyr641) Antibody

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STAT6 (pTyr641) Antibody gallery image 1

Western blot analysis of extracts from HeLa cells untreated or treated with IL-4 using Rabbit anti STAT6 (pTyr641) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with IL-4 (100ng/ml) for 30 minutes

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STAT6 (pTyr641) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti STAT6 (pTyr641) antibody

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  • Rabbit anti STAT6 (pTyr641)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2690P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2690
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti STAT6 (pTyr641) antibody recognizes STAT6, also known as IL-4 Stat when phosphorylated at tyrosine 641. STAT6 is a member of the STAT family of transcription factors. STATs reside in the cytosol until activated. Upon activation STATs dimerize and translocate to the nucleus where they exert their transcriptional effects. STAT6 is phosphorylated at tyrosine 641 by Janus associated kinases (JAKs) leading to dimerization and relocation to the nucleus (Wurster et al. 2000).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to STAT6 (pTyr641) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Phospho specific-peptide corresponding to residues surrounding tyrosine 641 of human STAT6
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      signal transducer activity
      nucleus
      cytosol
      sequence-specific DNA binding transcription factor activity
      protein binding
      calcium ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional STAT6 (pTyr641) Antibody Formats

    Formats Applications Sizes available
    STAT6 (pTyr641) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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