STAT4 (pTyr693) Antibody

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STAT4 (pTyr693) Antibody gallery image 1

Western blot analysis of extracts from HepG2 cells untreated or treated with IL4 using Rabbit anti STAT4 (pTyr693) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with IL4 (100ng/ml) for 30 minutes

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STAT4 (pTyr693) Antibody gallery image 2

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti STAT4 (pTyr693) antibody

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  • Rabbit anti STAT4 (pTyr693)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2687P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2687
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti STAT4 (pTyr693) antibody recognizes STAT4 when phosphorylated at tyrosine 693. STAT4 is a member of the STAT4 family of transcription factors. STAT4 is phosphorylated at tyrosine 693 by JAKs leading to homodimerzation and translocation to the nucleus (Wurster et al. 2000).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to STAT4 (pTyr693) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Phospho specific-peptide corresponding to residues surrounding tyrosine 693 of human STAT4
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      calcium ion binding
      sequence-specific DNA binding transcription factor activity
      cytoplasm
      nucleus
      JAK-STAT cascade
      regulation of transcription, DNA-dependent
      signal transducer activity
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/100
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional STAT4 (pTyr693) Antibody Formats

    Formats Applications Sizes available
    STAT4 (pTyr693) Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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