STAT3 (pSer727) Antibody

STAT3 (pSer727) Antibody gallery image 1

Western blot analysis of extracts from HepG2 cells untreated or treated with PMA using Rabbit anti STAT3 (pSer727) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with PMA (200 nM) for 30 minutes

  • STAT3 (pSer727) Antibody thumbnail image 1
  • Rabbit anti STAT3 (pSer727)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2685WBdatasheet pdfdatasheet pdf50 µl
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti STAT3 (pSer727) antibody recognizes signal transducer and activator of transcription 3 (STAT3), also known as acute-phase response factor, when phosphorylated at serine 727. STAT3 is a member of the STAT family of transcription factors, playing a key role in numerous processes including cell differentiation, proliferation and transformation. Phosphorylation of STAT3 at tyrosine 705 leads to homo- or heterodimerization of STAT3 and is a critical phosphorylation site for STAT3 activity (Huang et al. 2014).

      Serine 727 is also a key phosphorylation site of STAT3. Serine 727 is phosphorylated by multiple kinases including mitogen-activated kinases (MAPK), mammalian target of rapamycin kinase (mTOR), cyclin-dependent kinase 5 (CDK5) and nemo-like kinase (NLK) (Wakahara et al. 2012). Controversy exists around the effect of phosphorylation at serine 727 (Wakahara et al. 2012). There are reports that the transcriptional activity of STAT3 coincides with phosphorylation at serine 727. Other reports show STAT3 activation independent of serine 727. The role of serine 727 is likely dependent on the larger cellular context.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/10001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional STAT3 (pSer727) Antibody Formats

    Formats Applications Sizes available
    STAT3 (pSer727) Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

              Further Reading

              Write your review

              You may also be interested in...