STAT1 Alpha Antibody (PrecisionAbTM Antibody)

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STAT1 Alpha Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with STAT1 alpha antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/50,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to STAT1 alpha (molecular weight 87 kDa)

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  • STAT1 Alpha Antibody with Control Lysate
  • Rabbit anti STAT1 Alpha
  • STAT1 Alpha Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    STAT1 alpha antibody recognizes signal transducer and activator of transcription 1-alpha/beta, also known as transcription factor ISGF-3 components p91/p84. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00008WBdatasheet pdfdatasheet pdf100 µl
      VPA00008
      VPA00008KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00008K
      VPA00008KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00008KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human STAT1 alpha antibody recognizes human signal transducer and activator of transcription 1-alpha also known as transcription factor ISGF-3 components p91/p84. STAT1 alpha is one of two isoforms generated by alternative splicing and encoded for by the STAT1 gene. STAT1 alpha is a ~87 kDa protein belonging to the transcription factor STAT family containing a single SH2 domain. Rabbit anti Human STAT1 alpha antibody recognizes epitopes in a carboxy-terminal region of STAT1 alpha not contained in the short isoform STAT1 beta.

        Members of the STAT family respond to cytokines and growth factors and are phosphorylated by receptor associated kinase to form homo- or hetero-dimers. These then translocate to the cell nucleus to act as transcription activators. STAT1 is activated by a number of different ligands including the interferon, gp130 and receptor tyrosine kinase families (Gerhartz et al. 1996). Mutations in he STAT1 gene lead to a number of immunological disorders including severe combined immunodeficiency phenotypes (Sharfe et al. 2014), or more selective immunodeficiencies to macrophagic (usually mycobacteria) bacteria, viruses or fungi depending on the mutation or abnormality involved (Boisson-Dupuis et al. 2012).

        Rabbit anti Human STAT1 alpha antibody recognizes human STAT1 alpha as a single band of ~87 kDa in multiple human cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell line lysates tested.
      • Target Species
        Human
      • Western Blotting
        Anti STAT1 alpha antibody detects a band of approximately 87 kDa in MCF7 cell lysates.
        Anti STAT1 alpha antibody detects a band of approximately 87 kDa in MCF7 cell lysates.
        Anti STAT1 alpha antibody detects a band of approximately 87 kDa in MCF7 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by ion exchange chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by ion exchange chromatography
        Rabbit polyclonal antibody purified by ion exchange chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by ion exchange chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by ion exchange chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Peptide corresponding to amino acids near the C-terminus of human STAT1 alpha
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg MCF7 lysate lyophilized in RIPA buffer.
        400μg MCF7 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P
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        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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