SRSF1 Antibody (PrecisionAbTM Antibody) | AB01/3A7

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SRSF1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/3A7 gallery image 1

Western blot analysis of whole cell lysates probed with SRSF1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to SRSF1 (molecular weight 33 kDa)

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  • SRSF1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/3A7 thumbnail image 1
  • SRSF1 Antibody with Control Lysate
  • Mouse anti SRSF1
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG2b
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00538WBdatasheet pdfdatasheet pdf100 µl
    VMA00538
    VMA00538KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00538KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human SRSF1 antibody recognizes the serine/arginine-rich splicing factor 1, also known as SR splicing factor 1, alternative-splicing factor 1, pre-mRNA-splicing factor SF2 P33 subunit.

      The SRSF1 gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for SRSF1. In addition, a pseudogene of SRSF1 has been found on chromosome 13 (provided by RefSeq, Sep 2010).

      Mouse anti Human SRSF1 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti SRSF1 detects a band of approximately 33 kDa in LNCaP cell lysates
      Anti SRSF1 detects a band of approximately 33 kDa in LNCaP cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli-derived recombinant human SRSF1 (amino acids 1 - 248)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg LNCaP lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      nuclear speck
      termination of RNA polymerase II transcription
      nucleotide binding
      cytoplasm
      spliceosomal complex
      RNA binding
      mRNA splice site selection
      mRNA 3'-end processing
      protein binding
      mRNA export from nucleus
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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