Sp1 Antibody

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Sp1 Antibody gallery image 1

Western blot analysis of HeLa nuclear extract probed with Rabbit anti Human Sp1 (AHP850) followed by SHEEP ANTI RABBIT IgG:HRP (STAR54)

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Sp1 Antibody gallery image 2

Detection of human Sp1 in whole cell lysates of HEK293T cells transfected with a human Sp1 construct (T) or mock transfected (E) using Rabbit anti Human Sp1 (AHP850) and other anti Sp1 antibodies for immunoprecipitation at 3.75 mcg/mg lysate. Subsequently, membranes were probed with Rabbit anti Human Sp1 antibody (AHP850) 0.04mg/ml for detection by Western Blotting

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  • Rabbit anti Human Sp1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP850IP, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP850
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Sp1 antibody recognizes human Transcription factor Sp1 (Sp1), also known as TSFP1. Sp1 is a 785 amino acid, ~95kDa member of the Sp1 C2H2-type zinc-finger protein family. containing 3 C2H2-type zinc finger motifs. Sp1 is a transcription regulator, involved in the regulation of multiple genes.

      Sp1 has been implicated in a range of cellular processes including cell cycle regulation, maintenance of gene activation during development (Marin et al. 1997), regulation of nucleic acid metabolism and preservation of chromatin structure at gene loci.

      The transcription factor Sp1 binds to GC-rich motifs within DNA, and has been shown to interact with other tissue-specific transcription factors to modulate the activities of a variety of promoters (Höller et al. 1988). Sp1 is ubiquitously expressed in cell nuclei, although the level of expression may vary between cell types (Resendes and Rosmarin 2004).

    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation1ug/mg protein4ug/mg protein
      Western Blotting1/2,5001/10,000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Sp1 Antibody Formats

    Formats Applications Sizes available
    Sp1 Antibody : Purified IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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