SMURF1 Antibody (PrecisionAbTM Antibody)

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  • SMURF1 Antibody with Control Lysate
  • Rabbit anti SMURF1
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    SMURF1 antibody recognizes E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 or hSMURF1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00234WBdatasheet pdfdatasheet pdf100 µl
      VPA00234
      VPA00234KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00234KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human SMURF1 antibody recognizes the E3 ubiquitin-protein ligase SMURF1, also known as SMAD-specific E3 ubiquitin-protein ligase 1, Smad ubiquitination regulatory factor 1 and hSMURF1.

        The SMURF1 gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Dec 2010).

        Rabbit anti Human SMURF1 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti SMURF1 detects a band of approximately 81 kDa in Ramos cell lysates
        Anti SMURF1 detects a band of approximately 81 kDa in Ramos cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Rabbit Ig fraction prepared by ammonium sulphate precipitation
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Ramos lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        negative regulation of BMP signaling pathway
        I-SMAD binding
        ectoderm development
        R-SMAD binding
        ubiquitin-protein ligase activity
        protein localization at cell surface
        ubiquitin-dependent SMAD protein catabolic process
        protein polyubiquitination
        receptor catabolic process
        cytosol
        transforming growth factor beta receptor signaling pathway
        positive regulation of protein ubiquitination
        cell differentiation
        BMP signaling pathway
        negative regulation of transforming growth factor beta receptor signaling pathway
        protein ubiquitination involved in ubiquitin-dependent protein catabolic process
        plasma membrane
        protein export from nucleus
        proteasomal ubiquitin-dependent protein catabolic process
        activin binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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