SMC1A Antibody (PrecisionAbTM Antibody) | AB01/4H4

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SMC1A Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/4H4 gallery image 1

Western blot analysis of whole cell lysates probed with SMC1A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 118 second exposure. Arrow points to SMC1A (molecular weight 155 kDa).

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  • SMC1A Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB01/4H4 thumbnail image 1
  • Mouse anti SMC1A
  • SMC1A Antibody with Control Lysate
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    SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00637WBdatasheet pdfdatasheet pdf100 µl
      VMA00637
      VMA00637KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00637KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human SMC1A antibody recognizes structural maintenance of chromosomes protein 1A, also known as SMC protein 1A, SMC-1-alpha or segregation of mitotic chromosomes 1.

        Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by SMC1A. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. SMC1A, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in SMC1A result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2013).

        Mouse anti Human SMC1A antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti SMC1A antibody detects a band of approximately 155 kDa in HEK293 cell lysates
        Mouse anti SMC1A antibody detects a band of approximately 155 kDa in HEK293 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E. coli-derived recombinant human SMC1A (aa 837-1233)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        response to radiation
        mitotic spindle organization
        mitotic metaphase/anaphase transition
        microtubule motor activity
        cell division
        cytoplasm
        mitotic prometaphase
        protein heterodimerization activity
        chromatin binding
        signal transduction in response to DNA damage
        ATP binding
        mitotic sister chromatid cohesion
        condensed chromosome kinetochore
        cohesin core heterodimer
        DNA repair
        nucleoplasm
        cell cycle checkpoint
        negative regulation of DNA endoreduplication
        meiosis
        condensed nuclear chromosome
        nuclear mRNA splicing, via spliceosome
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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