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SMAD4 Antibody

SMAD4 Antibody gallery image 1

Western blot analysis of rat kidney tissue lysate (lane 1), 3T3 cell lysate (lane 2) and Jurkat cell lysate (lane 3) probed with Rabbit anti SMAD4 antibody

  • SMAD4 Antibody thumbnail image 1
  • Rabbit anti SMAD4
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2345WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti SMAD4 antibody recognizes mothers against decapentaplegic homolog or SMAD4, a protein involved in the transforming growth factor beta (TGF-ß) signalling pathway. The SMAD4 protein interacts with other SMAD family members to form a protein complex, which then moves to the cell nucleus. In the nucleus, the SMAD protein complex binds to specific areas of DNA where they control the activity of particular genes and regulate cell growth and division.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      30% Glycerol
      0.5% Bovine Serum Albumin
      0.01% Thiomersal
    • Immunogen
      Synthetic peptide corresponding to amino acids residues around 281 of human SMAD4
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti SMAD4 antibody recognizes a band of approximately 68kDa in Jurkat, 3T3 and rat kidney lysates
    • Instructions For Use

    Additional SMAD4 Antibody Formats

    Formats Applications Sizes available
    SMAD4 Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • SMAD4 Antibody thumbnail image 1

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