SMAD2 Antibody

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Western blot analysis of SMAD2 expression in a HeLa cell lysate using Goat anti SMAD2 (AHP694)

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  • Goat anti Human SMAD2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP694WBdatasheet pdfdatasheet pdf0.1 mg
    AHP694
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human SMAD2 antibody recognizes human SMAD2, also known as MADH2 or Mothers against decapentaplegic homolog 2. SMAD2 is a 467 amino acid ~50kDa intracellular signaling protein responsible for mediating the signal from TGFβ. SMAD2 associates woth the TGFβ receptor via the SMAD anchor for receptor activation (SARA) and on binding of TGFβ to the receptor SMAD2 is phosphorylated and dissociates from SARA. SMAD2 then forms an association with another SMAD family member SMAD4, important for translocation to the nucleus where it binds to its target promoters and activates TGFβ induced pathways.

      There are two identified isoforms of SMAD2 with the short isoform lacking an internal sequence from amino acid 79 to amino acid 108. The immunogen sequence for Goat anti Human SMAD2 antibody is contained within this region and hence this antibody is only expected to recognize the long, canonical isoform of SMAD2..

      SMAD2 is expressed at high levels in placenta skeletal muscle and heart (Xuan et al. 2007)
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/2001/500

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP694 detects bands of approximately 45kDa and 50kDa in HeLa cell lysates. a minimum incubation time of 1 hour is recommended with this antibody
    • Instructions For Use

    Additional SMAD2 Antibody Formats

    Formats Applications Sizes available
    SMAD2 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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