Slug Antibody | 6A7

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Slug Antibody | 6A7 gallery image 1

Western blot analysis of SLUG expression in HeLa using Mouse anti Human SLUG:Azide Free (MCA3456Z)

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Slug Antibody | 6A7 gallery image 2

Immunofluorescence showing SLUG in HeLa cells using Mouse anti Human SLUG:Azide Free (MCA3456Z)

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  • Slug Antibody | 6A7 thumbnail image 1
  • Slug Antibody | 6A7 thumbnail image 2
  • Mouse anti Human Slug:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    6A7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3456ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3456Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Slug antibody, clone 6A7 recognizes human Zinc finger protein SNAI2, also known as Slug, SNAI2, Neural crest transcription factor Slug or Protein snail homolog 2. Slug is a 268 amino acid ~35 kDa transcriptional repressor involved in development and migration of neural crest cells.

      Mutations in the SNAI2 gene can lead to expression of Waardenburg syndrome 2D (WS2D) an autosomal dominant disorder manifested by deafness and pigmentary disturbances (Sánchez-Martín et al. 2002) or Piebald trait (PBT) another autosomal dominant pigmentation disorder characterized by patches of white skin and hair lacking melanocytes (Sánchez-Martín et al. 2003).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 97-170 of human SNAI2
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      ectoderm and mesoderm interaction
      transcription
      canonical Wnt receptor signaling pathway
      zinc ion binding
      DNA binding
      multicellular organismal development
      osteoblast differentiation
      negative regulation of transcription from RNA polymerase II promoter
      nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Slug Antibody Formats

    Formats Clone Applications Sizes available
    Slug Antibody : Preservative Free 6A7 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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