SLC33A1 Antibody | 3A4

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SLC33A1 Antibody | 3A4 gallery image 1

Western blot analysis of SLC33A1 expression in PC-12 using Mouse anti Human SLC33A1:Azide Free (MCA3612Z)

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  • Mouse anti Human SLC33A1:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    3A4
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3612ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA3612Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human SLC33A1 antibody, clone 3A4 recognizes human Acetyl-coenzyme A transporter 1, also known as SLC33A1 or Solute carrier family 33 member 1. SLC33A1 is a 549 amino acid ~60 kDa multipass endoplasmic reticulum transmembrane glycoprotein involved in O-acetylation of gangliosides and negatively regulating BMP signaling (Kanamori et al. 1997).

      Mutations of the SLC33A1 gene can result in development of the condition Spastic paraplegia 42, autosomal dominant (SPG42), a slowly progressive neurodegenerative disorder with limb weakness and balance difficulties (Lin et al. 2008). Mutations can also lead to Congenital cataracts, hearing loss, and neurodegeneration (CCHLND) a lethal autosomal recessive disorder characterized by congenital cataracts, psychomotor retardation and hearing loss accompanied by reduced serum ceruloplasmin and copper concentration (Huppke et al. 2012).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-70 of human SLC33A1
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cell death
      acetyl-CoA transporter activity
      endoplasmic reticulum membrane
      integral to plasma membrane
      Golgi membrane
      membrane fraction
      transmembrane transport
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional SLC33A1 Antibody Formats

    Formats Clone Applications Sizes available
    SLC33A1 Antibody : Preservative Free 3A4 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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