Siglec-9 antibody | K8
Mouse anti Human Siglec-9
- Product Type
- Monoclonal Antibody
- Clone
- K8
- Isotype
- IgG1
- Specificity
- Siglec-9
Antibody Quality and Performance Guarantee
| Mouse anti Human Siglec-9, Clone K8, also known as CD329, recognises Human sialic acid-binding Ig-like lectin 9, a single pass type I membrane protein belonging to the immunoglobulin superfamily. Human Siglec-9 is broadly expressed in a range of human tissues with high expression on monocytes and low-level expression on neutrophils and subpopulations of NK, B, and T cells. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant Siglec-9 fused to Fc region of human IgG.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the SP2 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/25 | 1/200 |
| Immunofluorescence | |
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| Immunoprecipitation | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- This product is suitable for use in indirect ELISA applications.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Siglec-9 antibody
-
Zhang, J.Q. et al. (2000) Siglec-9, a novel sialic acid binding member of the immunoglobulin superfamily expressed broadly on human blood leukocytes.
J Biol Chem. 275 (29): 22121-6. -
Avril, T. et al. (2004) The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells.
J Immunol. 173 (11): 6841-9. -
Ikehara, Y. et al. (2004) Negative regulation of T cell receptor signaling by Siglec-7 (p70/AIRM) and Siglec-9.
J Biol Chem. 279 (41): 43117-25.
- Synonyms
- CD329
- UniProt
- Q9Y336
- Entrez Gene
- SIGLEC9
- GO Terms
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007166 cell surface receptor linked signaling pathway
MCA5890GA
0911
161447
167832
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