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Siglec-5/Siglec-14 antibody | 1A5

Mouse anti Human Siglec-5/Siglec-14

Product Type
Monoclonal Antibody
Clone
1A5
Isotype
IgG1
Specificity
Siglec-5/Siglec-14

Product Code Applications Pack Size List Price Your Price Qty
MCA5787GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF IP 0.1 mg loader
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loader

Mouse anti Human Siglec-5/Siglec-14 antibody, clone 1A5 recognizes human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage.

Mouse anti Human Siglec-5/Siglec-14 antibody, clone 1A5, is one of several Siglec-5 antibodies which also recognises human Siglec-14 (Angata et al. 2006). Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species.

Siglec-5 is also related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation.

Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Mouse anti Human Siglec-5/Siglec-14 antibody, clone 1A5 cross reacts with Chimpanzee (Jaroenpool et al. 2007).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
>
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Description Mouse IgG1 Negative Control

References for Siglec-5/Siglec-14 antibody

  1. Cornish, A.L. et al. (1998) Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.
    Blood. 92 (6): 2123-32.
  2. Avril, T. et al. (2005) Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.
    J Biol Chem. 280 (20): 19843-51.
  3. Angata, T. et al. (2006) Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
    FASEB J. 20: 1964-1973.
  4. Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
    Proc Natl Acad Sci U S A. 103 (20): 7765-70.
  5. Jaroenpool, J. et al. (2007) Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates.
    Cell Immunol. 250 (1-2): 91-104.

Further Reading

  1. Crocker, P.R. (2005) Siglecs in innate immunity.
    Curr Opin Pharmacol. 5 (4): 431-7.

Flow Cytometry

Immunoprecipitation

RRID
AB_11152943
UniProt
O15389
Q08ET2
Entrez Gene
SIGLEC5
SIGLEC14
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0005886 plasma membrane
GO:0005515 protein binding

MCA5787GA

0811 157507

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