Sandwich ELISA analysis of albumin binding using Mouse anti Human albumin (0300-0080) as a capture reagent and biotinylated Sheep anti Human albumin (AHP1790) as a detection reagent with human albumin purified from urine (PHP149) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma sample is displayed at 30000 (Green) and 60000 (Red) fold dilutions
Purified human albumin is a 66 kDa protein that binds and transports steroids, fatty acids, metal ions and thyroid hormones. Albumin accounts for approximately 50% of blood serum protein.
Purified human serum albumin - lyophilised
Reconstitute with phosphate buffer pH>7.0 containing 0.15M NaCl Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Purified albumin from human urine.
>99% by SDS PAGE
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.