Serum Albumin

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Sandwich ELISA analysis of albumin binding using Mouse anti Human albumin (0300-0080) as a capture reagent and biotinylated Sheep anti Human albumin (AHP1790) as a detection reagent with human albumin purified from urine (PHP149) as antigen. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma sample is displayed at 30000 (Green) and 60000 (Red) fold dilutions

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  • Purified Human Albumin
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  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP149IYdatasheet pdfdatasheet pdf1 mg
    PHP149
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Purified human albumin is a 66 kDa protein that binds and transports steroids, fatty acids, metal ions and thyroid hormones. Albumin accounts for approximately 50% of blood serum protein.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified human serum albumin - lyophilised
    • Reconstitution
      Reconstitute with phosphate buffer pH>7.0 containing 0.15M NaCl
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
      Purified albumin from human urine.
    • Preservative Stabilisers
      None present
    • Purity
      >99% by SDS PAGE
    • Approx. Protein Concentrations
    • Molecular Weight
      66kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Ammonium bicarbonate
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Serum Albumin Formats

    Formats Applications Sizes available
    Serum Albumin : Purified IY 1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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