Goat anti Human SDF-1 beta antibody recognizes human SDF-1 beta, otherwise known as CXCL12b, a 72 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform.
SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.
Purified IgG - lyophilised
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Antisera to human SDF-1 beta were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Recombinant human SDF-1 beta.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
1. Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV infection. Seminars in Immunology 10: 179-185.
2. Stumm, R. and Hollt, V. (2007) CXC chemokine receptor 4 regulates neuronal migration and axonal pathfinding in the developing nervous system: implications for neuronal regeneration in the adult brain. J. Mol. Endocrinol. 38: 377-382.
3. Kucia, M. et al. (2005) Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: Pivotal role of the SDF-1-CXCR4 axis. Stem Cells. 23: 879-894.