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SDCBP antibody

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Anti SDCBP Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti SDCBP Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2
Anti SDCBP Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Goat anti Human SDCBP antibody (VPA00143) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 100 second exposure. Arrow points to SDCBP (molecular weight 32 kDa).
Anti SDCBP Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Immunoprecipitation of SDCBP from HeLa whole cell lysate. The Input lane is 10% input; the SDCBP-IP lane is the immunoprecipitation with the SDCBP antibody (2.5 µg, VPA00143); the IgG-IP lane is the immunoprecipitation with Goat polyclonal IgG(2.5 µg, PCP001). Western blot analysis was performed using SDCBP antibody (1/1,000, VPA00143) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP. Arrow points to SDCBP.

Goat anti SDCBP

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
SDCBP

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VPA00143
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Goat anti Human SDCBP antibody recognizes the syndecan-binding protein 1, also known as syntenin-1, melanoma differentiation-associated protein 9, MDA-9, pro-TGF-alpha cytoplasmic domain-interacting protein 18, TACIP18 and scaffold protein Pbp1.

Encoded by SDCBP, syntenin-1 was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Goat anti Human SDCBP antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti SDCBP detects a band of approximately 32 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence SLEDLKVDKVIQAQ-C, from the N Terminus of the protein sequence.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
O00560
Entrez Gene
SDCBP
GO Terms
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0005109 frizzled binding
GO:0005137 interleukin-5 receptor binding
GO:0005634 nucleus
GO:0005789 endoplasmic reticulum membrane
GO:0005856 cytoskeleton
GO:0005895 interleukin-5 receptor complex
GO:0005925 focal adhesion
GO:0006612 protein targeting to membrane
GO:0006930 substrate-dependent cell migration, cell extension
GO:0007268 synaptic transmission
GO:0007411 axon guidance
GO:0008093 cytoskeletal adaptor activity
GO:0042327 positive regulation of phosphorylation
GO:0042470 melanosome
GO:0045545 syndecan binding
GO:0046330 positive regulation of JNK cascade
GO:0046982 protein heterodimerization activity
GO:0047485 protein N-terminus binding

VPA00143

161214

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