RPS6KA3 Antibody (PrecisionAbTM Antibody)

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RPS6KA3 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with RPS6KA3 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 72 second exposure. Arrow points to RPS6KA3 (molecular weight 83 kDa)

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  • Mouse anti RPS6KA3
  • RPS6KA3 Antibody with Control Lysate
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    Mouse anti-human RPS6KA3 antibody recognizes ribosomal protein S6 kinase alpha-3, also known as MAP kinase-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, RSK-2, S6K-alpha-3, insulin-stimulated protein kinase 1, p90-RSK 3 or ribosomal S6 kinase 2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00459WBdatasheet pdfdatasheet pdf100 µl
      VMA00459
      VMA00459KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00459KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human RPS6KA3 antibody recognizes the ribosomal protein S6 kinase alpha-3, also known as MAP kinase-activated protein kinase 1b, insulin-stimulated protein kinase 1, p90-RSK 3 or ribosomal S6 kinase 2.

        RPS6KA3 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in RPS6KA3 gene have been associated with Coffin-Lowry syndrome (CLS) (provided by RefSeq, Jul 2008), additionally mutations in the RPS6KA3 gene have been associated with a non-syndromic mental retardation X-linked-19 (MRX19).

        Mouse anti Human RPS6KA3 antibody detects a band of 83 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti RPS6KA3 detects a band of approximately 83 kDa in A431 cell lysate
        Anti RPS6KA3 detects a band of approximately 83 kDa in A431 cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody isolated from ascites by saturated ammonium sulfate precipitation followed by dialysis against PBS
        20μl Mouse monoclonal antibody isolated from ascites by saturated ammonium sulfate precipitation followed by dialysis against PBS
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified His-tagged fragment of human RPS6KA3
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        magnesium ion binding
        synaptic transmission
        protein phosphorylation
        protein serine/threonine kinase activity
        toll-like receptor 4 signaling pathway
        stress-activated MAPK cascade
        toll-like receptor 1 signaling pathway
        toll-like receptor 2 signaling pathway
        axon guidance
        Toll signaling pathway
        toll-like receptor 3 signaling pathway
        skeletal system development
        nucleoplasm
        central nervous system development
        MyD88-dependent toll-like receptor signaling pathway
        nerve growth factor receptor signaling pathway
        cytosol
        MyD88-independent toll-like receptor signaling pathway
        ATP binding
        innate immune response
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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