RIG-I Antibody (PrecisionAbTM Antibody) | 6C1

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RIG-I Antibody (PrecisionAb<sup>TM</sup> Antibody) | 6C1 gallery image 1

Western blot analysis of whole cell lysates probed with RIG-I antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 180 second exposure. Arrow points to RIG-I (molecular weight 114 kDa)

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  • RIG-I Antibody (PrecisionAb<sup>TM</sup> Antibody) | 6C1 thumbnail image 1
  • Mouse anti RIG-I
  • RIG-I Antibody with Control Lysate
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    Mouse anti-human RIG-1 antibody recognizes the probable ATP-dependent RNA helicase DDX58, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, RNA helicase RIG-I or retinoic acid-inducible gene 1 protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00463WBdatasheet pdfdatasheet pdf100 µl
      VMA00463
      VMA00463KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00463KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human RIG-I antibody recognizes RIG-I, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide or retinoic acid-inducible gene 1 protein.

        DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX58 encodes a protein containing RNA helicase-DEAD box protein motifs and two caspase recruitment domains (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. (provided by RefSeq, Jul 2008).

        Mouse anti Human RIG-I antibody detects a band of 114 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti RIG-I detects a band of approximately 114 kDa in Jurkat cell lysate
        Anti RIG-I detects a band of approximately 114 kDa in Jurkat cell lysate
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from ascites
        20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant protein of human RIG-I produced in HEK293T cell
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        positive regulation of defense response to virus by host
        protein binding
        response to virus
        ATP-dependent helicase activity
        zinc ion binding
        positive regulation of interferon-beta production
        positive regulation of interferon-alpha production
        innate immune response
        RNA binding
        ATP binding
        negative regulation of type I interferon production
        cytosol
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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