Retinol Binding Protein 4 Antibody (PrecisionAbTM Antibody)

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Retinol Binding Protein 4 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with retinol binding protein 4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, #170-6516, Bio-Rad) and visualized on the ChemiDoc MP with 73 second exposure, Arrow points to retinol binding protein 4 (molecular weight 20 kDa)

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  • Retinol Binding Protein 4 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Retinol Binding Protein 4 Antibody with Control Lysate
  • Retinol Binding Protein 4 Antibody with Control Lysate
  • Mouse anti Retinol Binding Protein 4
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    Retinol binding protein 4 antibody recognizes retinol binding protein 4, also known as plasma retinol binding protein, PRBP or RBP. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00045WBdatasheet pdfdatasheet pdf100 µl
      VMA00045
      VMA00045KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00045K
      VMA00045KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00045KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human retinol binding protein 4 antibody recognizes human serum retinol binding protein 4 (RBP4), a 201 amino acid single-chain glycoprotein belonging to the lipocalin family, which is responsible for the transport of Retinol (vitamin A1) from the liver to peripheral target tissues.

        In plasma, the RBP4-retinol complex interacts with transthyretin preventing its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency and can cause night vision problems (Besch et al. 2003). RBP4 gene variants have been implicated in insulin resistance (Saucedo et al. 2014)

        Mouse anti Human retinol binding protein 4 antibody recognizes free RBP4 and RBP4 in complex with transthyretin. It reacts with apo- as well as holo-RBP4. Mouse anti Human RBP4 antibody recognizes RBP4 as a single band of ~20 kDa in HepG2 cell line whole cell lysates by western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti retinol binding protein 4 antibody detects a band of approximately 20 kDa in HepG2 cell lysates.
        Anti retinol binding protein 4 antibody detects a band of approximately 20 kDa in HepG2 cell lysates.
        Anti retinol binding protein 4 antibody detects a band of approximately 20 kDa in HepG2 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Human recombinant retinol binding protein 4
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        female genitalia morphogenesis
        embryonic organ morphogenesis
        extracellular space
        positive regulation of insulin secretion
        retinal binding
        retinol transporter activity
        response to retinoic acid
        embryonic retina morphogenesis in camera-type eye
        vagina development
        retinol binding
        cardiac muscle tissue development
        urinary bladder development
        positive regulation of immunoglobulin secretion
        glucose homeostasis
        heart trabecula formation
        uterus development
        maintenance of gastrointestinal epithelium
        embryonic skeletal system development
        lung development
        protein binding
        negative regulation of cardiac muscle cell proliferation
        gluconeogenesis
        retinol metabolic process
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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