Retinol Binding Protein 4 Antibody (PrecisionAbTM Antibody)

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Retinol Binding Protein 4 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with retinol binding protein 4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, #170-6516, Bio-Rad) and visualized on the ChemiDoc MP with 73 second exposure, Arrow points to retinol binding protein 4 (molecular weight 20 kDa)

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  • Retinol Binding Protein 4 Antibody with Control Lysate
  • Mouse anti Retinol Binding Protein 4
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    Retinol binding protein 4 antibody recognizes retinol binding protein 4, also known as plasma retinol binding protein, PRBP or RBP. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00045WBdatasheet pdfdatasheet pdf100 µl
      VMA00045
      VMA00045KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00045KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human retinol binding protein 4 antibody recognizes human serum retinol binding protein 4 (RBP4), a 201 amino acid single-chain glycoprotein belonging to the lipocalin family, which is responsible for the transport of Retinol (vitamin A1) from the liver to peripheral target tissues.

        In plasma, the RBP4-retinol complex interacts with transthyretin preventing its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency and can cause night vision problems (Besch et al. 2003). RBP4 gene variants have been implicated in insulin resistance (Saucedo et al. 2014)

        Mouse anti Human retinol binding protein 4 antibody recognizes free RBP4 and RBP4 in complex with transthyretin. It reacts with apo- as well as holo-RBP4. Mouse anti Human RBP4 antibody recognizes RBP4 as a single band of ~20 kDa in HepG2 cell line whole cell lysates by western blotting under reducing conditions.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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